New London, CT
The impact of stress has been extensively studied in cell culture systems however most of these models have been conducted in single cell environments (neurons or astrocytes or microglia). While important, these systems do not fully capture the physiological environment of the brain, including the important interactions between different cellular groups.We designed a model to study neuronal differentiation in a co-culture system using immortalized cells. The predominant approach to co-culture systems involves utilizing primary stem cells derived from embryonic mouse brains to investigate molecular pathways and gene expressions. The use of immortalized cells will allow us to increase the sample consistency, cost, accessibility, and significantly reducing the need for live animal usage.
Omur, Nartsav, "Designing a Physiologically Relevant Cell Culture Model to Examine the Impact of Stress on Neural Differentiation" (2023). All Internship Reflections. 2427.
The views expressed in this paper are solely those of the author.